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BactoSiA™ by OptraSCAN

AI-Assisted TB Detection
& Bacillary Grading

Combining high-resolution whole-slide scanning with trained AI algorithms to detect, quantify, and grade acid-fast bacilli — making Tuberculosis detection faster, more consistent, and accessible in any laboratory setting.

~99.65% Detection Accuracy
WHO-Aligned Grading
2–4 Minute Slide-to-Report
300+ FOV per Slide
Powered by OS-SiA™
AI-Assisted TB Detection Platform

One integrated workflow. Smear to graded report
in 2–4 minutes.

BactoSiA combines high-resolution brightfield scanning with trained AI algorithms to automatically detect and quantify acid-fast bacilli on Ziehl–Neelsen-stained sputum samples — making TB detection faster, more consistent, and accessible in any laboratory setting.

Digitize Any Volume

Works with OS-SiX, OS-Lite, OS-Ultra, or OS-FLi. Standard smear preparation — no special protocols required.

AI-Assisted Triage

Classifies slides as likely positive or likely negative. 300+ fields of view analyzed per slide with ~99.65% accuracy.

WHO-Aligned Grading

Confirmed positive smears graded using standardized criteria — Scanty, 1+, 2+, 3+ — for consistent reporting.

Digital Review & Tracking

All results feed into IMAGEPath® for review, reporting, quality assurance, and longitudinal treatment monitoring.

How BactoSiA Works

Scan. Index. Analyze.
Slide to report in minutes.

Powered by OS-SiA™ — the world's first patented technology that scans, indexes, and analyzes simultaneously.

01
Scan
Smear slides prepared using standard protocols are digitized on OptraSCAN systems (OS-SiX, OS-Lite, OS-Ultra, or OS-FLi) to generate high-resolution whole-slide images within a scanner-native workflow.
02
Index
Indexed thumbnails, slide maps, and metadata are automatically generated during acquisition. Slides are categorized, catalogued, and made searchable — tagged regions enable organized case management without manual sorting.
03
Analyze
AI-assisted triage classifies slides as likely positive or likely negative. Positive smears are graded using WHO-aligned criteria (Scanty, 1+, 2+, 3+). All results feed directly into IMAGEPath® for review, reporting, and longitudinal tracking.
Brightfield Smear Overview

From raw sample to annotated report —
every step digitized.

BactoSiA processes Ziehl–Neelsen-stained sputum smears through four distinct stages, each producing digital outputs for review and quality assurance.

FOV Generated

300+ fields of view automatically captured and mapped across the entire smear area.

Clear FOV

High-resolution imagery reveals cellular detail with optimized focus and contrast.

Visible Bacilli with Annotations

AI-highlighted regions pinpoint detected AFB with visual markers for rapid pathologist review.

Software Output Panel

Comprehensive digital report with grading, FOV summary, and exportable results via IMAGEPath®.

The Global TB Challenge

Why smear microscopy needs
a digital upgrade.

Tuberculosis remains one of the world's deadliest infectious diseases. Laboratories in high-burden regions face mounting pressure to process more samples with fewer resources — while maintaining consistent, reliable results.

10M+

New Cases Annually

Tuberculosis affects over 10 million people each year globally, straining laboratory capacity in the most affected regions.

100+

Fields Reviewed Per Slide

Manual AFB smear microscopy requires pathologists to review 100+ fields of view per slide — tedious, time-intensive, and prone to variability.

30%

Reader Variability

Inter-reader variability in smear grading remains a significant challenge, impacting treatment decisions and follow-up protocols.

How BactoSiA Addresses This

Faster processing without additional trained personnel

Consistent, WHO-standardized grading across readers and sites

Digital workflows that integrate into existing lab infrastructure

Actionable results at the point of acquisition — not downstream

Built for High-Volume Labs

Designed for scale
and operational efficiency.

BactoSiA integrates scanning, indexing, and AI-assisted analysis into a single workflow — supporting laboratories that manage high testing volumes and repeat evaluations.

Streamlined Workflows

Digitize and analyze within a single process — no downstream bottlenecks or separate analysis stations needed.

Reduced Reader Variability

AI-assisted grading brings consistency across readers and sites, supporting standardized evaluation practices.

Longitudinal Tracking

Digital records support treatment monitoring and follow-up with structured, searchable case histories.

Existing Infrastructure

Operates within current lab staffing models and workflows — no additional infrastructure required.

Use Cases

Where BactoSiA delivers
the most impact.

Purpose-built for the settings where TB detection matters most — from national reference labs to mobile field units.

Public Health Laboratories

Rapid AFB smear review, WHO-standardized grading, and structured reporting for population-level TB screening programs at national and regional scale.

Mid/High-Volume TB Labs

Boost throughput and standardization for routine smear assessment, treatment monitoring, and follow-up — reducing manual workload without adding staff.

Mobile Units & NGOs

Smear-based triage and remote expert review in field settings with limited infrastructure. Digitized slides enable remote consultation from anywhere.

Telepathology Networks

Remote QA, second reads, and consistent bacillary grading across distributed laboratory sites — supported by OptraSCAN's TELEPath platform.

Compatible Scanners

Works with every
OptraSCAN system.

OS-SiX™
6-Slide Brightfield
OS-Lite™
15-Slide Brightfield
OS-Ultra™
80–480 Slide Brightfield
OS-FLi™
Fluorescence + Brightfield

Transform your TB lab with BactoSiA.

See how AI-assisted AFB detection and WHO-aligned grading can modernize your smear microscopy workflow — slide to report in minutes.